Gibco

Human Apo-SAA1 Recombinant Protein, PeproTech®

Product Details

300-53-100UG

Applications

Tested Dilution

Publications

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Virus

Expression System

E. coli

Amino acid sequence

MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY

Molecular weight

11.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

300-53-1MG will be provided as 2 x 500 µg (300-53-500UG).

Recombinant Human Apo-SAA1 is an 11.7 kDa protein containing 105 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SAA suppresses α-PD-1 induced anti-tumor immunity by driving T_H2 polarization in lung adenocarcinoma.

Cell death & disease

Wang X,Wen S,Du X,Zhang Y,Yang X,Zou R,Feng B,Fu X,Jiang F,Zhou G,Liu Z,Zhu W,Ma R,Feng J,Shen B

300-53 was used in In vitro experiments to indicate that cancer stem cells secreted SAA have significant biological impact to promote treatment resistant and tumor fibrosis by driving cancer stemness transformation and type 2 immunity polarization via P2X7 receptor.

Sat Nov 04 00:00:00 EDT 2023

Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.

Nature communications

Han Y,Tomita T,Kato M,Ashihara N,Higuchi Y,Matoba H,Wang W,Hayashi H,Itoh Y,Takahashi S,Kurita H,Nakayama J,Okumura N,Hiratsuka S

300-53 was used in In vitro experiments to demonstrate that post-translational citrullination of fibrinogen creates a metastatic niche in the vulnerable spots.

Thu Aug 24 00:00:00 EDT 2023

Subversion of Serotonin Receptor Signaling in Osteoblasts by Kynurenine Drives Acute Myeloid Leukemia.

Cancer discovery

Galán-Díez M,Borot F,Ali AM,Zhao J,Gil-Iturbe E,Shan X,Luo N,Liu Y,Huang XP,Bisikirska B,Labella R,Kurland I,Roth BL,Quick M,Mukherjee S,Rabadán R,Carroll M,Raza A,Kousteni S

300-53 was used in In vivo experiments to investigate the mechanisms involved in acute myeloid leukemia using patient-derived xenografts, and patient samples.

Fri Apr 01 00:00:00 EDT 2022

Effects of serum amyloid protein A on influenza A virus replication and viral interactions with neutrophils.

Journal of leukocyte biology

White MR,Hsieh IN,De Luna X,Hartshorn KL

Thu Jul 01 00:00:00 EDT 2021

SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.

iScience

Getachew A,Abbas N,You K,Yang Z,Hussain M,Huang X,Cheng Z,Tan S,Tao J,Yu X,Chen Y,Yang F,Pan T,Xu Y,Xu G,Zhuang Y,Wu F,Li Y

Fri May 21 00:00:00 EDT 2021

Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

Communications biology

Klooster JPT,Bol-Schoenmakers M,van Summeren K,van Vliet ALW,de Haan CAM,van Kuppeveld FJM,Verkoeijen S,Pieters R

300-53 was used in In vitro experiments to indicate that IL22 contributes to the innate responses against both bacteria and viruses.

Thu May 27 00:00:00 EDT 2021

Dysfunctional high-density lipoprotein activates toll-like receptors via serum amyloid A in vascular smooth muscle cells.

Scientific reports

Schuchardt M,Prüfer N,Tu Y,Herrmann J,Hu XP,Chebli S,Dahlke K,Zidek W,van der Giet M,Tölle M

Mon Mar 04 00:00:00 EST 2019

COOH-terminal SAA1 peptides fail to induce chemokines but synergize with CXCL8 and CCL3 to recruit leukocytes via FPR2.

Blood

De Buck M,Gouwy M,Berghmans N,Opdenakker G,Proost P,Struyf S,Van Damme J

Thu Jan 25 00:00:00 EST 2018

Bacterial Lipoproteins Constitute the TLR2-Stimulating Activity of Serum Amyloid A.

Journal of immunology (Baltimore, Md. : 1950)

Burgess EJ,Hoyt LR,Randall MJ,Mank MM,Bivona JJ,Eisenhauer PL,Botten JW,Ballif BA,Lam YW,Wargo MJ,Boyson JE,Ather JL,Poynter ME

Mon Oct 15 00:00:00 EDT 2018

Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins.

Journal of lipid research

Thomas SE,Harrison EH

Sat Oct 01 00:00:00 EDT 2016

Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.

European journal of immunology

Gouwy M,De Buck M,Pörtner N,Opdenakker G,Proost P,Struyf S,Van Damme J

300-53 was used in In vitro experiments to demonstrate that the serum amyloid A (SAA)-α isoform also chemoattracts monocyte-derived immature dendritic cells (DCs) in the Boyden and µ-slide chemotaxis assay and that its chemotactic activity for monocytes and DCs was indirectly mediated via rapid chemokine induction.

Thu Jan 01 00:00:00 EST 2015

Inflammatory remodeling of the HDL proteome impairs cholesterol efflux capacity.

Journal of lipid research

Vaisar T,Tang C,Babenko I,Hutchins P,Wimberger J,Suffredini AF,Heinecke JW

Sat Aug 01 00:00:00 EDT 2015

Serum amyloid A1α induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils.

Journal of leukocyte biology

De Buck M,Berghmans N,Pörtner N,Vanbrabant L,Cockx M,Struyf S,Opdenakker G,Proost P,Van Damme J,Gouwy M

Tue Dec 01 00:00:00 EST 2015

Serum amyloid A1α induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils.

Journal of leukocyte biology

De Buck M,Berghmans N,Pörtner N,Vanbrabant L,Cockx M,Struyf S,Opdenakker G,Proost P,Van Damme J,Gouwy M

Tue Dec 01 00:00:00 EST 2015

Serum amyloid A and inflammation in diabetic kidney disease and podocytes.

Laboratory investigation; a journal of technical methods and pathology

Anderberg RJ,Meek RL,Hudkins KL,Cooney SK,Alpers CE,Leboeuf RC,Tuttle KR

Sun Mar 01 00:00:00 EST 2015

Ex vivo and in vitro effect of serum amyloid a in the induction of macrophage M2 markers and efferocytosis of apoptotic neutrophils.

Journal of immunology (Baltimore, Md. : 1950)

Sun L,Zhou H,Zhu Z,Yan Q,Wang L,Liang Q,Ye RD

300-53 was used in In vivo experiments to suggest a potential role for SAA to alter macrophage phenotype and modulate macrophage functions through an MyD88-dependent mechanism that involves IRF4-mediated transcription.

Fri May 15 00:00:00 EDT 2015

Extracellular acidosis is a novel danger signal alerting innate immunity via the NLRP3 inflammasome.

The Journal of biological chemistry

Rajamäki K,Nordström T,Nurmi K,Åkerman KE,Kovanen PT,Öörni K,Eklund KK

Fri May 10 00:00:00 EDT 2013

Endogenous Acute Phase Serum Amyloid A Lacks Pro-Inflammatory Activity, Contrasting the Two Recombinant Variants That Activate Human Neutrophils through Different Receptors.

Frontiers in immunology

Christenson K,Björkman L,Ahlin S,Olsson M,Sjöholm K,Karlsson A,Bylund J

300-53 was used in In vitro experiments to study whether endogenous acute phase serum amyloid A (SAA) mediates pro-inflammatory effects.

Tue Apr 30 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: amyloid a; MGC111216; SAA; serum amyloid A protein; Serum amyloid A-1 protein; Serum amyloid A1 protein; Serum Amyloid-A; tumor protein p53 inducible protein 4

Human Apo-SAA1 Recombinant Protein, PeproTech®

Product Details

300-53-100UG

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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