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Product Details
P2947
Product Specifications
Species
Human
Expression System
Insect cells
Amino acid sequence
TQHGIRLPLR
Molecular weight
55 kDa
Class
Recombinant
Type
Protein
Purity
85% as determined by a Coomassie® blue stained SDS-PAGE gel.
Activity
609 Units/mg. One unit is defined as the amount of BACE1 required to cleave one pmol of Rhodamine-EVNLDAEFK-Quencher in one minute at room temperature. Cleavage of the FRET peptide is monitored by an increase in fluorescence (Ex535 nm/ Em590 nm) in the following assay reaction: 250 nM Rhodamine-EVNLDAEFK-Quencher in 50 mM Sodium Acetate (pH 4.5) in a total volume of 30 µL accoding to the BACE1 (βSecretase) FRET Assay Kit, Red (P2985).
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
proprietary buffer
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Target Information
BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; Memapsin; Memapsin-2; Memapsin2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
Gene Aliases: ASP2; BACE; BACE1; HSPC104; KIAA1149
UniProt ID: (Human) P56817
Entrez Gene ID: (Human) 23621
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