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Invitrogen
Human BAFF (BLyS) Recombinant Protein
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Product Details
14-8943-80
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human BAFF, amino acids Ala134-Leu285 (Accession # Q9Y275)
Molecular weight
18.5 kDa
Class
Recombinant
Type
Protein
Purity
>90% by reducing SDS-PAGE
Endotoxin concentration
<0.40 ng/µg
Activity
ED50 = 1.6 µg/mL; determined by a mouse splenocyte survival assay.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity.
Applications Reported: Recombinant human BAFF is biologically active.
Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6 µg/mL.
Source: E. coli expressed amino acids Ala134-Leu285 accession # Q9Y275.
Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6 µg/mL.
Endotoxin: Less than 0.40 ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE.
Molecular Weight: 18.5 kDa.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4
Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4
UniProt ID: (Human) Q9Y275
Entrez Gene ID: (Human) 10673
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