Gibco

Human BRAK (CXCL14) Recombinant Protein, PeproTech®

Human BRAK (CXCL14) Protein in Functional Assay (FN)

FIGURE: 1 / 1

Human BRAK (CXCL14) Protein (300-50-1MG) in FN

Bioassay analysis of Human BRAK (CXCL14) Recombinant Protein, PeproTech® (Product # 300-50-1MG).

Product Image Gallery

Product Details

300-50-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Immunostaining (IS)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse

Expression System

E. coli

Amino acid sequence

SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

Molecular weight

9.4 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract activated monocytes using a concentration range of 1.0-10.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human BRAK is a 9.4 kDa protein containing 77 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.

The Journal of biological chemistry

Lee AW,Deruaz M,Lynch C,Davies G,Singh K,Alenazi Y,Eaton JRO,Kawamura A,Shaw J,Proudfoot AEI,Dias JM,Bhattacharya S

Fri Jul 19 00:00:00 EDT 2019

Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.

Cancer cell

Costa A,Kieffer Y,Scholer-Dahirel A,Pelon F,Bourachot B,Cardon M,Sirven P,Magagna I,Fuhrmann L,Bernard C,Bonneau C,Kondratova M,Kuperstein I,Zinovyev A,Givel AM,Parrini MC,Soumelis V,Vincent-Salomon A,Mechta-Grigoriou F

Mon Mar 12 00:00:00 EDT 2018

Genetically engineered two-warhead evasins provide a method to achieve precision targeting of disease-relevant chemokine subsets.

Scientific reports

Alenazi Y,Singh K,Davies G,Eaton JRO,Elders P,Kawamura A,Bhattacharya S

Fri Apr 20 00:00:00 EDT 2018

CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract.

Acta histochemica et cytochemica

Suzuki H,Yamada K,Matsuda Y,Onozuka M,Yamamoto T

Tue Dec 26 00:00:00 EST 2017

CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract.

Acta histochemica et cytochemica

Suzuki H,Yamada K,Matsuda Y,Onozuka M,Yamamoto T

Tue Dec 26 00:00:00 EST 2017

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

CXCL14-Like Immunoreactivity Exists in Somatostatin-Containing Cells of Mouse Pancreas.

Acta histochemica et cytochemica

Suzuki H,Yamamoto T

300-50 was used in Immunohistochemistry to study the distribution of the chemokine CXCL14 in the mouse pancreas using immunohistochemical techniques to show that CXCL14, secreted from somatostatin-containing cells, may modulate insulin secretion in a paracrine fashion, and play a novel role in glucose homeostasis in addition to its well-known chemotactic activities.

Fri Dec 25 00:00:00 EST 2015

Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection.

Journal of translational medicine

Zeng J,Yang X,Cheng L,Liu R,Lei Y,Dong D,Li F,Lau QC,Deng L,Nice EC,Xie K,Huang C

Mon Jan 07 00:00:00 EST 2013

Constitutive and inflammation-dependent antimicrobial peptides produced by epithelium are differentially processed and inactivated by the commensal Finegoldia magna and the pathogen Streptococcus pyogenes.

Journal of immunology (Baltimore, Md. : 1950)

Frick IM,Nordin SL,Baumgarten M,Mörgelin M,Sørensen OE,Olin AI,Egesten A

Sat Oct 15 00:00:00 EDT 2011

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

CXCL14 deficiency in mice attenuates obesity and inhibits feeding behavior in a novel environment.

PloS one

Tanegashima K,Okamoto S,Nakayama Y,Taya C,Shitara H,Ishii R,Yonekawa H,Minokoshi Y,Hara T

Fri Apr 23 00:00:00 EDT 2010

SpeB of Streptococcus pyogenes differentially modulates antibacterial and receptor activating properties of human chemokines.

PloS one

Egesten A,Olin AI,Linge HM,Yadav M,Mörgelin M,Karlsson A,Collin M

Mon Jun 15 00:00:00 EDT 2009

Disruption of CXC motif chemokine ligand-14 in mice ameliorates obesity-induced insulin resistance.

The Journal of biological chemistry

Nara N,Nakayama Y,Okamoto S,Tamura H,Kiyono M,Muraoka M,Tanaka K,Taya C,Shitara H,Ishii R,Yonekawa H,Minokoshi Y,Hara T

Fri Oct 19 00:00:00 EDT 2007

A chemokine-binding domain in the tumor necrosis factor receptor from variola (smallpox) virus.

Proceedings of the National Academy of Sciences of the United States of America

Alejo A,Ruiz-Argüello MB,Ho Y,Smith VP,Saraiva M,Alcami A

Tue Apr 11 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: bolekine; breast and kidney; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 14; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXC; CXC chemokine in breast and kidney; CXC motif chemokine; CXCL; MGC10687; MIP-2 gamma; MIP-2G; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK); Small-inducible cytokine B14; tumor-suppressing chemokine

View more View less

Gene Aliases: BMAC; BRAK; CXCL14; KEC; KS1; MIP-2g; MIP2G; NJAC; PSEC0212; SCYB14; UNQ240/PRO273

Human BRAK (CXCL14) Recombinant Protein, PeproTech®

Human BRAK (CXCL14) Protein (300-50-1MG) in FN

Product Details

300-50-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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