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Product Details
150-19T-100UG
Product Specifications
Species
Human
Expression System
CHO cells
Amino acid sequence
SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GGEKGEFREH HHHHHHH
Tag
His-tag
Molecular weight
51.6 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
PeproTech's CHO cell-derived Recombinant Human Tissue Factor is a homodimeric glycoprotein comprised of the 227-amino-acid length monomer containing a C-terminal His-Tag and has a calculated molecular weight of 51.6 kDa. Due to glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 40-50 kDa by SDS-PAGE gel under reducing conditions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD142; Coagulation factor III; coagulation factor III (thromboplastin, tissue factor); TF; Thromboplastin; Tissue factor
Gene Aliases: CD142; F3; TF; TFA
UniProt ID: (Human) P13726
Entrez Gene ID: (Human) 2152
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