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Invitrogen
Human CD200 His-tag Recombinant Protein
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Product Details
A42571
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD200, amino acidds Met1-Gly232 (Accession # NP_005935.4) with a C-terminal His-tag
Tag
His-tag
Molecular weight
24 kDa
Class
Recombinant
Type
Protein
Purity
≥98 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized Human CD200 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind CD200R Protein, Human, Recombinant (His & hFc Tag), the EC50 is 5-28 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; OX-2 membrane glycoprotein; unnamed protein product
Gene Aliases: CD200; MOX1; MOX2; MRC; My033; OX-2
UniProt ID: (Human) P41217
Entrez Gene ID: (Human) 4345
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