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Product Details
RP-87999
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD3D, Met1-Ala105 (Accession # P04234-1) with a C-terminal His-tag hIgG1 Fc region and human CD3E, Met1-Asp126 (Accession # P07766-1) with a C-terminal Flag-tag hIgG Fc region were co-expressed and purified as a human CD3D & CD3E heterodimer
Tag
Fc-tag
Molecular weight
44-54 kDa
Class
Recombinant
Type
Protein
Purity
≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (200 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
CD3E and CD3D Heterodimer Protein is produced by mammalian expression system and are involved in mediating signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD3 antigen, delta subunit; CD3 delta; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); OKT3, delta chain; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain
Gene Aliases: CD3-DELTA; CD3D; CD3E; IMD18; IMD19; T3D; T3E; TCRE
UniProt ID: (Human) P04234, (Human) P07766
Entrez Gene ID: (Human) 915, (Human) 916
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