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Invitrogen
Human CD38 His-tag Recombinant Protein
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Product Details
A42570
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD38, amino acids Val43-Ile300 (Accession # NP_001766.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
31.3 kDa
Class
Recombinant
Type
Protein
Purity
>97 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide
(NGD+) to cyclic GDPribose.The specific activity is > 2, 500 pmoles/min/µg.
2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.
3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind Human CD38 His-tag Recombinant Protein with an affinity constant of 5.429 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
(NGD+) to cyclic GDPribose.The specific activity is > 2, 500 pmoles/min/µg.
2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.
3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind Human CD38 His-tag Recombinant Protein with an affinity constant of 5.429 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Target Information
CD38 is a multifunctional ectoenzyme and type II transmembrane glycoprotein involved in various cellular processes. It catalyzes the conversion of NAD into secondary messengers such as nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR). CD38 plays a crucial role in B cell development, with expression levels fluctuating from high in immature cells to low in intermediate ones, and back to high in mature B cells. It is also present in a variety of tissues and hematopoietic cells, including T cells, NK cells, and monocytes, and is used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population is considered to define the most pluripotent hematopoietic stem cells. In addition to its surface expression, CD38 has been identified in the nucleus, where it may regulate calcium levels. It functions as a multi-catalytic ectoenzyme, serving roles as an ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and possibly NAD+ glycohydrolase, or as a cell surface receptor. CD38 is involved in the activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It also participates in cell adhesion, signal transduction, and calcium signaling. Antibodies to CD38 are valuable for subtyping lymphomas and leukemias, detecting plasma cells (such as in myelomas), and marking activated B and T cells. CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions in CD38 are associated with diseases like chronic lymphocytic leukemia and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; lymphocyte differentiation antigen CD38; NAD(+) nucleosidase; NAD+ nucleosidase; T10; unnamed protein product
Gene Aliases: ADPRC 1; ADPRC1; cADPR1; CD38
UniProt ID: (Human) P28907
Entrez Gene ID: (Human) 952
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