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Invitrogen
Human CD4 His-tag Recombinant Protein
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Product Details
A42543
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD4, amino acids Met1-Trp390 (Accession # NP_000607.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
42.2 kDa
Class
Recombinant
Type
Protein
Purity
≥90 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1.Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 µg/mL, 100 µL/well), approximately >40% will adhere specifically.
2.Immobilized Ibalizumab-Derived Anti-CD4 Antibody, Human IgG4 at 2 µg/mL (100 µL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag), the EC50 is 0.6-2.5 ng/mL(QC tested).
2.Immobilized Ibalizumab-Derived Anti-CD4 Antibody, Human IgG4 at 2 µg/mL (100 µL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag), the EC50 is 0.6-2.5 ng/mL(QC tested).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Gene Aliases: CD4; CD4mut
UniProt ID: (Human) P01730
Entrez Gene ID: (Human) 920
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