Invitrogen

Human CDC7/DBF4, GST Tag Recombinant Protein

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

PV6276

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

1-574

Tag

GST-tag

Molecular weight

91.2-103.5 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

The gene accession number for the subunits (CD7/DBF4) are NP_003494.1 and NP_006707.1.

Target Information

CDC7, or Cell Division Cycle 7, is a serine/threonine kinase that plays a crucial role in the initiation of DNA replication. It is essential for the activation of replication origins during the S phase of the cell cycle.

ASK, also known as Activator of S Phase Kinase/DBF4, is a regulatory subunit that binds to CDC7 and activates its kinase activity. The binding of ASK to CDC7 is necessary for the proper functioning of CDC7, as it regulates the activation and specificity of the kinase.

Together, the CDC7/ASK complex is critical for the regulation of DNA replication. This complex phosphorylates components of the pre-replication complex at replication origins, thereby promoting the initiation of DNA synthesis. By ensuring that replication origins fire at the appropriate time, the CDC7/ASK complex helps to maintain genomic stability and proper cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activator of S phase kinase; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; Chiffon homolog A; DBF4 homolog; DBF4 zinc finger A; DBF4-type zinc finger-containing protein 1; Protein DBF4 homolog A; zinc finger, DBF-type containing 1

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Gene Aliases: ASK; CDC7; CDC7L1; CHIF; DBF4; DBF4A; HsCDC7; Hsk1; huCDC7; ZDBF1