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Invitrogen
Human CDK1/Cyclin B, His Tag Recombinant Protein
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Product Details
PV3980
Product Specifications
Species
Human
Expression System
Insect cells
Amino acid sequence
Full length
Tag
His-tag
Molecular weight
91 kDa
Class
Recombinant
Type
Protein
Purity
80% by SDS-PAGE
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
20mM tris, pH 7.5, with 0.5mM EDTA, 2mM DTT, 150mM NaCl, 0.01% Triton X-100, 20% glycerol
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Protein Form: Recombinant, Full Length
Target Information
Cyclin-dependent kinase 1 (CDK1) and its regulatory partner Cyclin B are fundamental to the control of cell cycle transitions, specifically the G2/M phase transition, which ultimately leads to cell division. CDK1, a serine/threonine kinase, forms a complex with Cyclin B necessary for the initiation of mitosis. This complex phosphorylates various substrates to facilitate structural changes required for mitosis. The CDK1/Cyclin B complex is tightly regulated through a series of phosphorylation and dephosphorylation events that control its activation state. The nuclear-cytoplasmic shuttling of Cyclin B1, coupled with CDK1 activity, is crucial for proper mitotic entry and progression. Interestingly, CDK1/Cyclin B1 also plays a role in the coordination of mitochondrial bioenergetics, indicating a broader role in cell metabolism and division. Allosteric modifications, such as acetylation of specific lysine residues, notably lysine-33, also influence CDK1/Cyclin B interaction and function, emphasizing the intricate regulatory mechanisms governing their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; Cyclin-dependent kinase 1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; p34 protein kinase
Gene Aliases: CCNB; CCNB1; CDC2; CDC28A; CDK1; CDKN1; P34CDC2
UniProt ID: (Human) P14635, (Human) P06493
Entrez Gene ID: (Human) 891, (Human) 983
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