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Invitrogen
Human CDK3/cyclin E1, GST Tag Recombinant Protein
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Product Details
PV6300
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
1-305
Tag
GST-tag
Molecular weight
61.3-73.4 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
The gene accession number for the subunits CDK3 and Cyclin E1 are NP_001249.1 and NP_001229.1.
Target Information
CDK3, or Cyclin-Dependent Kinase 3, is a member of the cyclin-dependent kinase family involved in regulating cell cycle progression. Although less well-studied than some other CDKs, CDK3 is believed to play a role in the transition from the G1 phase to the S phase of the cell cycle.
Cyclin E is a regulatory subunit that binds to CDK3 and activates its kinase activity. The binding of Cyclin E to CDK3 is crucial for the proper functioning of CDK3, as cyclins regulate the activation and specificity of CDKs.
Together, the CDK3/Cyclin E complex contributes to the regulation of the cell cycle by phosphorylating target proteins that are essential for the G1 to S phase transition, thereby promoting DNA replication and cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 3; Cyclin-dependent kinase 3; G1/S-specific cyclin-E1
Gene Aliases: CCNE; CCNE1; CDK3; CDKN3; pCCNE1
UniProt ID: (Human) P24864, (Human) Q00526
Entrez Gene ID: (Human) 898, (Human) 1018
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