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Invitrogen
Human DIRAS3 (aa 154-229) Control Fragment Recombinant Protein
Product Details
RP-94376
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SDDTHREVALNDGATCAMEWNCAFMEISAKTDVNVQELFHMLLNYKKKPTTGLQEPEKKSQMPNTTEKLLDKCIIM
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (45%), Rat (45%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-55872. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
This gene is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DIRAS family GTP binding RAS like 3; DIRAS family, GTP-binding RAS-like 3; Distinct subgroup of the Ras family member 3; GTP-binding protein Di-Ras3; ras homolog gene family, member I; Rho-related GTP-binding protein RhoI
Gene Aliases: ARHI; DIRAS3; NOEY2; RHOI
UniProt ID: (Human) O95661
Entrez Gene ID: (Human) 9077
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