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Human EGF Protein (PHG0311) in Bioactivity
Immunofluorescence analysis of signal transducer and activator of transcription 3 (STAT3) was performed using A549 cells treated with 100 ng/mL human EGF recombinant protein (Product # PHG0311) for 30 minutes. The cells were labeled with phospho-STAT3 (Ser727) recombinant rabbit monoclonal antibody (Product # MA5-33199) (Panel a & c; Green). The composite (b & d) represents the merged with DAPI (blue) and F-actin (red) staining. Cells treated with EGF displayed upregulated phospho-STAT3 (Ser727) in comparison with untreated cells. The imag... View More
Product Details
PHG0311
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
E. coli
Molecular weight
6.2 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA
Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor
Gene Aliases: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
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