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Invitrogen
Human EGFR Fc Chimera Recombinant Protein
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Product Details
A42489
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human EGFR, amino acids Met1-Ser645 (Accession # NP_005219) with a C-terminal human IgG1 Fc-tag
Tag
Fc-tag
Molecular weight
95 kDa
Class
Recombinant
Type
Protein
Purity
>97 % as determined by SDS-PAGE. >85% as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human EGF at 10 µg/mL (100 µL/well) can bind human EGFR with a linear range of 0.64-400 ng/mL.
Immobilized recombinant human EGF at 10 µg/mL (100 µL/well) can bind human EGFR with a linear range of 0.64-400 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% trehalose, 0.01% Tween 80, 5% mannitol
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone
Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61
UniProt ID: (Human) P00533
Entrez Gene ID: (Human) 1956
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