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          • Proteins & Peptides ›
          • EGFR (d746-750 Mutant) Proteins

          Invitrogen

          Human EGFR [d746-750], GST Tag, His Tag Recombinant Protein

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          Cite Human EGFR [d746-750], GST Tag, His Tag Recombinant Protein

          Product Details

          PV6179

          Applications
          Tested Dilution
          Publications

          Kinase Assay (KA)

          Assay-dependent
          -
          Product Specifications

          Species

          Human

          Expression System

          Baculovirus

          Amino acid sequence

          672-1210

          Tag

          GST-tag, His-tag

          Molecular weight

          88.6 kDa

          Class

          Recombinant

          Type

          Protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          See Label

          Purification

          purified

          Storage buffer

          50mM HEPES, pH 7.5, with 20% glycerol, 5mM DTT, 100mM NaCl, 15mM glutathione

          Contains

          no preservative

          Storage conditions

          -80°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Dry ice

          Product Specific Information

          For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

          Human EGF-R, C-terminal fragment, amino acids H672-A1210 (as in GenBank entry NM_005228.3), amino acids 746-750 deleted, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

          • Validated in LanthaScreen™ Kinase Binding Assay
          • Protocols available for simple use and optimized performance
          • Multiple pack sizes available for convenient use

          Related Product:
          LanthaScreen™ Eu Kinase Binding Assay

          Protein Form: Recombinant

          Target Information

          EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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