Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Human EphA2 His-tag Recombinant Protein
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
RP-88050
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human EPHA2, amino acids Met1-Asn534 (Accession # P29317) with a C-terminal His-tag
Tag
His-tag
Molecular weight
65-75 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE and ≥90% as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 His at 2 µg/ml (100 µL/well) can bind Human Ephrin-A1 His and hFc, the EC50 of Human Ephrin-A1 His and hFc is 4.0-20.0 ng/mL.
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
purified
Storage buffer
20mM tris, pH 7.5, with 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK
Gene Aliases: ARCC2; CTPA; CTPP1; CTRCT6; ECK; EPHA2
UniProt ID: (Human) P29317
Entrez Gene ID: (Human) 1969
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support