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Invitrogen
Human Estrogen Receptor beta Recombinant Protein
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Product Details
RP-311
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Expression System
Insect cells
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
Lot Specific
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
RP-311 contains 180 µg of full-length ER Beta in 50 mM bis tris propane (pH 9.0), 500 mM KCl, 50% glycerol, 275 mM Urea, 0.6% w/v CHAPS, and 2 mM DTT.
This recombinant protein was obtained from Baculovirus-infected Sf9 cells and is both hormone binding and estrogen response element binding competent. RP-311 may be used as a control in Western blot experiments with PA1-311 or for gel shift and super shift assays with an appropriate estrogen response element and antibody (PA1-311 can be used for supershift experiments with vitellogenin ERE).
RP-311 calculated molecular weight is 53.4 kDa.
Target Information
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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