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Invitrogen
Human FGF basic Recombinant Protein
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Product Details
68-8785-82
Product Specifications
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Dry ice
Product Specific Information
Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Fibroblast Growth Factor basic (FGF-b, also known as FGF-2) is a member of the FGF family, a highly conserved family of 16-34 kDa heparin-binding proteins. FGF-b exists in several isoforms, and although they are equally active, only the 18 kDa form is secreted while the 23 kDa form localizes to the nucleus. It lacks the signal sequence peptide necessary for the ER/Golgi pathway, indicating that secretion occurs via an alternate pathway. FGF-b shares four common tyrosine kinase receptors, FGFR 1-4, and require the binding of a second surface protein, the ubiquitously expressed heparan sulfate proteoglycan, in order to fully activate these receptors. FGF family members affect the proliferation, differentiation, mobility, and survival of several cell types, including fibroblasts, osteoblasts, smooth muscle cells, and neuroblasts. FGF-b expression has been detected in several cell types, including fibroblasts, macrophages, endothelial cells, epithelial cells, and neurons. FGF-b is particularly important in embryonic development as triggers of neurogenesis, angiogenesis, and neovascularization and has most recently been studied for its ability to maintain the proliferation of embryonic stem cell cultures in an undifferentiated state. Some members of the family, including FGF-b, remain active during adulthood and play a role in bone formation and tissue repair. FGF family members are also implicated in many types of cancer and may contribute to tumor vascularization.
Applications Reported: SAFE™ Human FGF basic Recombinant Animal Free Protein is biologically active.
Applications Tested: The ED50 of this protein, as measured by proliferation assay of NIH3T3 mouse fibroblast cells, is less than or equal to 0.2 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Source: Plant expressed 146 amino acids (Pro143-Ser288) with 10 amino acid N terminal tag-accession NP_001997.5.
Bioactivity: The ED50 of this protein, as measured by proliferation assay of NIH3T3 mouse fibroblast cells, is less than or equal to 0.2 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE.
Molecular Weight: 17.8 kDa.
Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile PBS to a concentration no less than 100 µg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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