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Invitrogen
Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Recombinant Protein
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Product Details
RP-87977
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1, with natural variant Phe 176 Val) with a C-terminal His-tag
Tag
His-tag
Molecular weight
44.2 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
BLI:
1. Loaded Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on His1K Biosensor, can bind
Bevacizumab (IgG1) with an affinity constant of 0.83 µM as determined in a BLI assay (Sartorius Octet Red384).
2. Loaded Recombinant Human FcγRIIIA / CD16a (V176) Protein, His Tag (Cat. No. 10389-H08H1) on His1K Biosensor, can
bind Bevacizumab (IgG1) with an affinity constant of 0.81 µM as determined in BLI assay (Sartorius Octet RED384) (QC
tested).
SPR:
1. Captured Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on AntiHis Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 µM as determined in an SPR assay (Biacore T200).
2. Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-
H08H1) with an affinity constant of 0.78 µM as determined in an SPR assay (Biacore T200).
1. Loaded Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on His1K Biosensor, can bind
Bevacizumab (IgG1) with an affinity constant of 0.83 µM as determined in a BLI assay (Sartorius Octet Red384).
2. Loaded Recombinant Human FcγRIIIA / CD16a (V176) Protein, His Tag (Cat. No. 10389-H08H1) on His1K Biosensor, can
bind Bevacizumab (IgG1) with an affinity constant of 0.81 µM as determined in BLI assay (Sartorius Octet RED384) (QC
tested).
SPR:
1. Captured Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-H08H1) on AntiHis Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 µM as determined in an SPR assay (Biacore T200).
2. Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein (Cat. 10389-
H08H1) with an affinity constant of 0.78 µM as determined in an SPR assay (Biacore T200).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (4000 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1
Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20
UniProt ID: (Human) P08637, (Human) O75015
Entrez Gene ID: (Human) 2214, (Human) 2215
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