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Human Follistatin Recombinant Protein, PeproTech®

Human Follistatin Protein in Functional Assay (FN)

FIGURE: 1 / 1

Human Follistatin Protein (120-13-100UG) in FN

Bioassay analysis of Human Follistatin Recombinant Protein, PeproTech® (Product # 120-13-1MG).

Product Image Gallery

Product Details

120-13-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 10 publications 10 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Chicken, Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN

Molecular weight

31.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 ug/ml in the presence of 7.5 ng/ml Activin A.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Follistatin is a 31.5 kDa protein containing 288 amino acids. Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elevated circulating follistatin associates with an increased risk of type 2 diabetes.

Nature communications

Wu C,Borné Y,Gao R,López Rodriguez M,Roell WC,Wilson JM,Regmi A,Luan C,Aly DM,Peter A,Machann J,Staiger H,Fritsche A,Birkenfeld AL,Tao R,Wagner R,Canouil M,Hong MG,Schwenk JM,Ahlqvist E,Kaikkonen MU,Nilsson P,Shore AC,Khan F,Natali A,Melander O,Orho-Melander M,Nilsson J,Häring HU,Renström E,Wollheim CB,Engström G,Weng J,Pearson ER,Franks PW,White MF,Duffin KL,Vaag AA,Laakso M,Stefan N,Groop L,De Marinis Y

Wed Nov 10 00:00:00 EST 2021

Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis.

Cell reports

Yucer N,Ahdoot R,Workman MJ,Laperle AH,Recouvreux MS,Kurowski K,Naboulsi DJ,Liang V,Qu Y,Plummer JT,Gayther SA,Orsulic S,Karlan BY,Svendsen CN

120-13 was used in Cell Culture to generate induced pluripotent stem cells (iPSCs) from healthy individuals and young ovarian cancer patients with germline pathogenic BRCA1 mutations (BRCA1mut).

Tue Dec 28 00:00:00 EST 2021

A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming.

EMBO molecular medicine

Cangkrama M,Wietecha M,Mathis N,Okumura R,Ferrarese L,Al-Nuaimi D,Antsiferova M,Dummer R,Innocenti M,Werner S

Tue Apr 07 00:00:00 EDT 2020

A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming.

EMBO molecular medicine

Cangkrama M,Wietecha M,Mathis N,Okumura R,Ferrarese L,Al-Nuaimi D,Antsiferova M,Dummer R,Innocenti M,Werner S

Tue Apr 07 00:00:00 EDT 2020

The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis.

Scientific reports

Griffiths R,Woods S,Cheng A,Wang P,Griffiths-Jones S,Ronshaugen M,Kimber SJ

Mon Mar 16 00:00:00 EDT 2020

Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium.

Scientific reports

Yucer N,Holzapfel M,Jenkins Vogel T,Lenaeus L,Ornelas L,Laury A,Sareen D,Barrett R,Karlan BY,Svendsen CN

Wed Sep 06 00:00:00 EDT 2017

Activin A as a mediator of NK-dendritic cell functional interactions.

Journal of immunology (Baltimore, Md. : 1950)

Seeger P,Bosisio D,Parolini S,Badolato R,Gismondi A,Santoni A,Sozzani S

Sat Feb 01 00:00:00 EST 2014

Activin A promotes neuronal differentiation of cerebrocortical neural progenitor cells.

PloS one

Rodríguez-Martínez G,Molina-Hernández A,Velasco I

Tue Jan 29 00:00:00 EST 2013

Growth differentiation factor 9 (GDF9) suppresses follistatin and follistatin-like 3 production in human granulosa-lutein cells.

PloS one

Shi FT,Cheung AP,Huang HF,Leung PC

Tue Dec 27 00:00:00 EST 2011

Dynamic interaction networks in a hierarchically organized tissue.

Molecular systems biology

Kirouac DC,Ito C,Csaszar E,Roch A,Yu M,Sykes EA,Bader GD,Zandstra PW

Tue Oct 05 00:00:00 EDT 2010

INHBA overexpression promotes cell proliferation and may be epigenetically regulated in esophageal adenocarcinoma.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Seder CW,Hartojo W,Lin L,Silvers AL,Wang Z,Thomas DG,Giordano TJ,Chen G,Chang AC,Orringer MB,Beer DG

120-13 was used in In vitro experiments to show that INHBA is overexpressed in EAC relative to dysplastic and BM tissue. INHBA overexpression may promote cell proliferation and may be affected by promoter demethylation and histone acetylation in EAC cell lines.

Wed Apr 01 00:00:00 EDT 2009

Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin.

BMC developmental biology

Edqvist PH,Lek M,Boije H,Lindbäck SM,Hallböök F

Fri Apr 25 00:00:00 EDT 2008

Structural basis for the inhibition of activin signalling by follistatin.

The EMBO journal

Harrington AE,Morris-Triggs SA,Ruotolo BT,Robinson CV,Ohnuma S,Hyvönen M

Wed Mar 08 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Activin-binding protein; Follictatin; Follistatin; follistatin isoform FST317; FS

Human Follistatin Recombinant Protein, PeproTech®

Human Follistatin Protein (120-13-100UG) in FN

Product Details

120-13-100UG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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