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Invitrogen
Human GM-CSF Recombinant Protein
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Product Details
68-8777-63
Product Specifications
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Dry ice
Product Specific Information
Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.
Applications Reported: SAFE™ Human GM-CSF Recombinant Animal Free Protein is biologically active.
Applications Tested: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Source: Plant expressed 127 amino acids (Ala18-Glu144). accession NP_000749.2.
Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than 0.2 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE.
Molecular Weight: 26 kDa with glycosylation.
Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile reagent grade deionized water to a concentration no less than 100 µg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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