Invitrogen

Human GSK-3A, GST Tag Recombinant Protein

Product Details

PR9188A

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

78.4 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT, 50% glycerol

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
• Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

GSK3A for the LanthaScreen Kinase Binding Assay

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
• Tracer 236 (Cat No. PV5592)
• Eu-labeled GST Antibody (Cat No. PV5594)

Related Link
• Learn more about the LanthaScreen™ Eu Binding Assay.

Protein Form: Full Length

Target Information

Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686D0638; Glycogen synthase kinase-3 alpha; GSK-3 alpha; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A