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Human Heat Stable bFGF Protein (PHG0369) in Bioactivity
bFGF-target and off-target gene expression was comparable in cells treated with native bFGF and Heat Stable bFGF. The TaqMan Array Human Signal Transduction Pathways array (Product # 4418775) and the TaqMan Array Human FGF Pathway (Product # 4414136) were used to assess expression of genes in the androgen, calcium, CREB, estrogen, hedgehog, insulin, JAK-STAT, LDL, mitogenic, NFAT, NFκB, p53, phospholipase C... View More
Product Details
PHG0369
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Bovine,
Human,
Mouse
Expression System
E. coli
Amino acid sequence
155 aa + 20 aa for N-terminal tag for purification purposes
Molecular weight
19 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<1 EU/µg
Activity
ED50 = 0.6 - 1.1 ng/mL; determined by the dose-dependent proliferation of mouse NIH/3T3 fibroblast cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM potassium phosphate with 750mM NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 1.0 mg/mL. Alternatively, reconstitute at lower concentrations in carrier-containing medium. Do not vortex. Apportion the reconstituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in medium, or buffered solution containing carrier protein, such as PBS with 0.1% BSA. For the 5 µg unit size, reconstitute directly in carrier-containing medium. For other unit sizes, reconstitute to 1.0 mg/mL as follows:
• 50 µg, reconstitute in 50 µL
• 100 µg, reconstitute in 100 µL
• 500 µg, reconstitute in 500 µL
Storage: Store the lyophilized protein at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C (not in a frost-free freezer) for up to 12 months. Avoid repeated freeze-thaw cycles.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; FGF-2; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); Fibroblast growth factor basic; H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b; prostatropin
Gene Aliases: BFGF; FGF-2; FGF2; FGFB; HBGF-2
UniProt ID: (Human) P09038
Entrez Gene ID: (Human) 2247
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