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Invitrogen
Human Histone H1.1 (aa 116-154) Control Fragment Recombinant Protein
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Product Details
RP-100392
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPK
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (55%), Rat (55%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111188. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1 histone family, member 1; histone 1, H1a; Histone H1.1; Histone H1a
Gene Aliases: H1-1; H1.1; H1A; H1F1; HIST1; HIST1H1A
UniProt ID: (Human) Q02539
Entrez Gene ID: (Human) 3024
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