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Product Details
RP-10931
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IGF-1 recombinant protein contains 70 amino acids
Molecular weight
7.6 kDa
Class
Recombinant
Type
Protein
Purity
>98% by RP-HPLC and SDS-PAGE
Endotoxin concentration
ng/µg
Activity
ED50 < 1.0 ng/mL; determined by the dose-dependent proliferation of mouse Balb/c 3T3 cells.
Conjugate
Form
Lyophilized
Purification
Chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This is a recombinant protein suitable for use as a control in various applications.
Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.
Reconstitute in sterile water not less than 100 µg/mL.
Target Information
IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin C; Somatomedin-C
Gene Aliases: IBP1; IGF-1; IGF-I; IGF1; IGFI; MGF
UniProt ID: (Human) P05019
Entrez Gene ID: (Human) 3479
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