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Invitrogen
Human IL-17A Recombinant Protein
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Product Details
RIL1720
Product Specifications
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
RIL1720 contains a mixture of non-glycosylated form (15.5 kD) and N-linked glycosylated form (20 kD) with 136 amino acid residues.
Purity: > 95% pure by SDS-PAGE and FPLC analyses.
Activity: Active at 1 ng/mL concentration as determined by the dose-dependent production of IL-6 by normal human dermal fibroblasts.
Use: IL-17 exerts activity in the range of 1 to 100 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 20 ng/µL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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