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Invitrogen
Human IL-2 Recombinant Protein
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Product Details
14-8029-81
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
50 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Human IL-2 (also known as TCGF) is a ~15 kDa factor produced mainly by activated CD4^+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.
Applications Reported: Recombinant human IL-2 is biologically active and can promote proliferation of T lymphocytes in culture. It is also used as standard in human IL-2 ELISA.
Applications Tested: The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.
Source: E. coli-expressed amino acids Ala 21-Thr 153, accession #NM_000586.
Bioactivity: The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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