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Invitrogen
Human IL-4 Recombinant Protein
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Product Details
68-8780-82
Product Specifications
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Amount
100 µg
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Dry ice
Product Specific Information
Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.
Applications Reported: SAFE™ Human IL-4 Recombinant Animal Free Protein is biologically active.
Applications Tested: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.2 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Source: Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. accession number NP_000580.
Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7 ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg.
Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE.
Molecular Weight: 17.2 kDa (apparent 23 and 24 kDa).
Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100 µg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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