Search
Additional Information:
{{banner.description}}
Product Details
RIL4I
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
P. pastoris
Amino acid sequence
Human IL-4, amino acids 25-153 (Accession # P05112)
Molecular weight
15 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Activity
Determined by the proliferation of human TF-1 cells.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Amount
20 µg
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Mature human IL-4 was expressed in Pichia pastoris, X-33. GenBank Accession # P05112, amino acids 25 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLQEI. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant human IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; IL-4v2; IL4; ILN; Interleukin; interleukin 4; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support