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Invitrogen
Human IL-6 Recombinant Protein
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Product Details
14-8069-80
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Human IL-6 (also known as B Cell Stimulating Factor BCSF, BSF-2) is a ~21-28 kDa factor produced by a variety of cell types including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences antigen-specific immune response, inflammatory response, and the development of neurons and is a major mediator of the acute phase reaction.
Applications Reported: Recombinant human IL-6 is biologically active and can promote proliferation of human cell line in culture. It is also used as standard in human IL-6 ELISA.
Applications Tested: This recombinant human IL-6 has been tested by sandwich ELISA using anti-human IL-6 antibody pair (MQ2-13A5/MQ2-39C3) and in bioassays using human cell line TF-1. The ED50 measured in a TF-1 proliferation assay is typically less than 0.10 ng/mL, corresponding to a specific activity of greater than 1x10e7 Units/mg.
Source: E. coli expressed amino acids Pro 29-Met 212 of mature human IL-6 accession # AF372214.
Bioactivity: Measured by human TF-1 cell proliferation assay. The ED50 is 0.1 ng/mL, corresponding to a specific activity of 1.0 x 10e7 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The methionylated N-terminal protein has a predicted molecular mass of 21,041. The DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides on SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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