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Invitrogen
Human KappaB ras1 (aa 54-80) Control Fragment Recombinant Protein
Product Details
RP-107172
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KEQLHLYDTRGLQEGVELPKHYFSFAD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66503. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
KappaB ras-1 (kappa-B-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with Ikappa-B (Ikappa-B), an inhibitor of the transcription factor NF-kappa-B. Ikappa-B exists in two homologous forms, Ikappa-B-alpha and Ikappa-B-beta, although Ikappa-B-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive Ikappa-B-alpha-NF-kappa-B complexes can shuttle in and out of the nucleus, Ikappa-B-beta-NF-kappa-B complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the Ikappa-B-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of Ikappa-B-beta and regulating the rate of degradation of Ikappa-B-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C15orf1; I-kappa-B-interacting Ras-like protein 1; I1PP2A; kappa B-ras 1; Kappa B-Ras protein 1; MAPM; MGC119787; MGC150373; NF-kappa-B inhibitor-interacting Ras-like protein 1; NFKB inhibitor interacting Ras-like 1; NFKB inhibitor interacting Ras-like protein 1; PHAP1; PP32
Gene Aliases: kappaB-Ras1; KBRAS1; NKIRAS1
UniProt ID: (Human) Q9NYS0
Entrez Gene ID: (Human) 28512
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