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Invitrogen
Human LCK, HIS Tag Recombinant Protein
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Product Details
P3043
Product Specifications
Species
Human
Expression System
Insect cells
Tag
His-tag
Molecular weight
60.6 kDa
Class
Recombinant
Type
Protein
Purity
70% as determined by a SDS-PAGE
Activity
240 nM of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 2 µg/mL
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris, pH 7.5, with 100mM NaCl, 0.05mM EDTA, 0.05% NP-40, 1mM DTT, 50% glycerol
Contains
no preservative
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Specific Activity: 240 nmoles of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein at 30°C.
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
Gene Aliases: IMD22; LCK; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
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