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Invitrogen
Human M-CSF Recombinant Protein
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Product Details
14-8789-80
Product Specifications
Expression System
Insect cells
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Human Macrophage Colony Stimulating Factor (M-CSF, also called CSF-1) is a ~36 kDa disulfide-linked homodimer produced by a variety of cells including fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF regulates macrophage survival, proliferation and differentiation. M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages.
Applications Reported: Recombinant human M-CSF is biologically active and can promote proliferation of M-NFS-60 cells in culture.
Applications Tested: The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS-60 cells, is 3 ng/mL. This corresponds to a specific activity of 3.3 X 10e5 Units/mg.
Source: Human M-CSF Glu34-Ser190 accession # NM-172211 expressed in insect cells with a C-terminal 6X His tag.
Bioactivity: The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS-60 cells, is 3 ng/mL. This corresponds to a specific activity of 3.3 X 10e5 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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