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Invitrogen
Human PD-1 His-tag Recombinant Protein
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Product Details
A42533
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human PD1, amino acids Met1-Gln167 (Accession # NP_005009.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
17.4 kDa
Class
Recombinant
Type
Protein
Purity
>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Immobilized Human PD-1 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL.
2. Immobilized Human PD-1 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Anti-PD1 (MDX)-IgG4 Antibody (Nivolumab), the EC50 is 8-60 ng/mL.
3. Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested).
4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 10.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
2. Immobilized Human PD-1 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) can bind Anti-PD1 (MDX)-IgG4 Antibody (Nivolumab), the EC50 is 8-60 ng/mL.
3. Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested).
4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 10.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
purified
Storage buffer
50mM MOPS, pH 7, with 5% mannitol, 500mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD279; CD279; PD-1; hPD1; PD-1; PD1; alternatively spliced; PDCD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2; unnamed protein product
Gene Aliases: AIMTBS; CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2
UniProt ID: (Human) Q15116
Entrez Gene ID: (Human) 5133
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