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Invitrogen
Human SULT2B1 (aa 192-269) Control Fragment Recombinant Protein
Product Details
RP-98895
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
HIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRR
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (81%), Rat (81%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59696. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Cytosolic sulfotransferases (STs or SULTs) catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. Her et al. (1998) identified an EST with homology to ST enzymes. By PCR with human placental and prostate cDNA as template, they isolated 2 alternatively spliced cDNAs, identical throughout most of their sequences, but having different 5-prime ends. The shorter cDNA, SULT2B1a, encodes a protein of 350 amino acids; the longer cDNA, SULT2B1b, encodes a 365-amino acid protein. Genomic PCR analysis revealed that the gene encoding both cDNAs, SULT2B1, contains 7 exons, with 2 alternative first exons being used to generate SULT2B1a and SULT2B1b. Northern blot analysis revealed that the SULT2B1 gene is expressed as a 1.4-kb transcript predominantly in prostate, placenta, and trachea.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alcohol sulfotransferase; Hydroxysteroid sulfotransferase 2; ST2B1; Sulfotransferase 2B1; Sulfotransferase family 2B member 1; Sulfotransferase family cytosolic 2B member 1; sulfotransferase family, cytosolic, 2B, member 1; SULT2B1a
Gene Aliases: HSST2; SULT2B1
UniProt ID: (Human) O00204
Entrez Gene ID: (Human) 6820
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