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Product Details
150-17H-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human,
Mouse
Expression System
CHO cells
Amino acid sequence
HHHHHHHHGK NNVPRLKLSY KEMLESNNVI TFNGLANSSS YHTFLLDEER SRLYVGAKDH IFSFDLVNIK DFQKIVWPVS YTRRDECKWA GKDILKECAN FIKVLKAYNQ THLYACGTGA FHPICTYIEI GHHPEDNIFK LENSHFENGR GKSPYDPKLL TASLLIDGEL YSGTAADFMG RDFAIFRTLG HHHPIRTEQH DSRWLNDPKF ISAHLISESD NPEDDKVYFF FRENAIDGEH SGKATHARIG QICKNDFGGH RSLVNKWTTF LKARLICSVP GPNGIDTHFD ELQDVFLMNF KDPKNPVVYG VFTTSSNIFK GSAVCMYSMS DVRRVFLGPY AHRDGPNYQW VPYQGRVPYP RPGTCPSKTF GGFDSTKDLP DDVITFARSH PAMYNPVFPM NNRPIVIKTD VNYQFTQIVV DRVDAEDGQY DVMFIGTDVG TVLKVVSIPK ETWYDLEEVL LEEMTVFREP TAISAMELST KQQQLYIGST AGVAQLPLHR CDIYGKACAE CCLARDPYCA WDGSACSRYF PTAKRATRAQ DIRNGDPLTH CSDLHHDNHH GHSPEERIIY GVENSSTFLE CSPKSQRALV YWQFQRRNEE RKEEIRVDDH IIRTDQGLLL RSLQQKDSGN YLCHAVEHGF IQTLLKVTLE VIDTEHLEEL LHKDDDGDGS KTKEMSNSMT PSQKVWYRDF MQLINHPNLN TMDEFCEQVW KRDRKQRRQR PGHTPGNSNK WKHLQENKKG RNRRTHEFER APRSVGGPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
Tag
Fc-tag, His-tag
Molecular weight
226.2 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
150-17H-1MG will be provided as 10 x 100 µg (150-17H-100UG).
150-17H-500UG will be provided as 5 x 100 µg (150-17H-100UG).
Recombinant Human Semaphorin 3A Fc has a calculated molecular weight of 226.2 kDa and therefore runs above the 200kDa marker by SDS-PAGE analysis under nonreducing conditions. When run under reducing conditions, this protein migrates as three distinct bands that, due to glycosylation, run higher than expected at apparent molecular weights of approximately 120-130 kDa, 90-100 kDa, and 35-40 kDa.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: collapsin 1; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Sema III; semaphorin D; Semaphorin III; semaphorin L; Semaphorin-3A
Gene Aliases: coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMA3A; SEMAD; SEMAIII; SEMAL; SemD
UniProt ID: (Human) Q14563
Entrez Gene ID: (Human) 10371
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