Gibco

Human Slit2-N Recombinant Protein, PeproTech®

Product Details

150-11-100UG

Applications

Tested Dilution

Publications

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

HEK293 cells

Amino acid sequence

QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNLPETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAFQ GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRVDAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSAKEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKITDIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS NRITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLLANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AIQDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIPRD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFSNMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGAF NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGPGEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEEDGFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGEHCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP PMV

Molecular weight

122.35 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

150-11-1MG will be provided as 5 x 200 µg (150-11-200UG).

150-11-500UG will be provided as multiple 100 µg (150-11-100UG) and 200 µg (150-11-200UG) vials.

Recombinant Human Slit2-N is a 1093 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor, and has a calculated, theoretical molecular weight of 122.35 kDa. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0 kDa by SDS-PAGE analysis under reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brief Report: Robo1 Regulates the Migration of Human Subventricular Zone Neural Progenitor Cells During Development.

Stem cells (Dayton, Ohio)

Guerrero-Cazares H,Lavell E,Chen L,Schiapparelli P,Lara-Velazquez M,Capilla-Gonzalez V,Clements AC,Drummond G,Noiman L,Thaler K,Burke A,Quiñones-Hinojosa A

150-11 was used in Cell Culture to indicate that the migration of human NPCs from the SVZ is partially regulated by the Slit-Robo axis.

Sat Jul 01 00:00:00 EDT 2017

A requirement for filopodia extension toward Slit during Robo-mediated axon repulsion.

The Journal of cell biology

McConnell RE,Edward van Veen J,Vidaki M,Kwiatkowski AV,Meyer AS,Gertler FB

Mon Apr 25 00:00:00 EDT 2016

The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.

Journal of immunology (Baltimore, Md. : 1950)

Mukovozov I,Huang YW,Zhang Q,Liu GY,Siu A,Sokolskyy Y,Patel S,Hyduk SJ,Kutryk MJ,Cybulsky MI,Robinson LA

Thu Oct 01 00:00:00 EDT 2015

The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.

Journal of immunology (Baltimore, Md. : 1950)

Mukovozov I,Huang YW,Zhang Q,Liu GY,Siu A,Sokolskyy Y,Patel S,Hyduk SJ,Kutryk MJ,Cybulsky MI,Robinson LA

Thu Oct 01 00:00:00 EDT 2015

The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.

Journal of immunology (Baltimore, Md. : 1950)

Mukovozov I,Huang YW,Zhang Q,Liu GY,Siu A,Sokolskyy Y,Patel S,Hyduk SJ,Kutryk MJ,Cybulsky MI,Robinson LA

Thu Oct 01 00:00:00 EDT 2015

Whole-exome and targeted sequencing identify ROBO1 and ROBO2 mutations as progression-related drivers in myelodysplastic syndromes.

Nature communications

Xu F,Wu LY,Chang CK,He Q,Zhang Z,Liu L,Shi WH,Guo J,Zhu Y,Zhao YS,Gu SC,Fei CM,Wu D,Zhou LY,Su JY,Song LX,Xiao C,Li X

Thu Nov 26 00:00:00 EST 2015

Low Expression of Slit2 and Robo1 is Associated with Poor Prognosis and Brain-specific Metastasis of Breast Cancer Patients.

Scientific reports

Qin F,Zhang H,Ma L,Liu X,Dai K,Li W,Gu F,Fu L,Ma Y

150-11 was used in Sample Preparation (Western Blotting) to investigate whether the Slit2/Robo1 axis has any role in brain metastasis of breast cancer.