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Product Details
300-59-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human,
Mouse
Expression System
E. coli
Amino acid sequence
AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY
Molecular weight
12 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Assay 1: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ug/ml. Assay 2: Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 ng/ml.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Human TFF-2 is a 12.0 kDa polypeptide of 107 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion. SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: SP; Spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1; Trefoil factor 2
Gene Aliases: SML1; SP; TFF2
UniProt ID: (Human) Q03403
Entrez Gene ID: (Human) 7032
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