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Human TGF-beta 3, Animal-Free Recombinant Protein, PeproTech®

Human TGF-beta 3, Animal-Free Protein in Functional Assay (FN)

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Human TGF-beta 3, Animal-Free Protein (AF-100-36E-10UG) in FN

WHO Comparison of Human TGF-beta 3, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-36E-1MG).

Product Image Gallery

Product Details

AF-100-36E-10UG

Applications

Tested Dilution

Publications

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Amphibian, Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Molecular weight

25 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by TGF-beta3's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≥ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human TGF-beta3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation.

Nature metabolism

Li L,Conradson DM,Bharat V,Kim MJ,Hsieh CH,Minhas PS,Papakyrikos AM,Durairaj AS,Ludlam A,Andreasson KI,Partridge L,Cianfrocco MA,Wang X

Wed Sep 01 00:00:00 EDT 2021

Robust neuronal differentiation of human iPSC-derived neural progenitor cells cultured on densely-spaced spiky silicon nanowire arrays.

Scientific reports

Harberts J,Siegmund M,Schnelle M,Zhang T,Lei Y,Yu L,Zierold R,Blick RH

Wed Sep 22 00:00:00 EDT 2021

Elevated IL-6R on CD4⁺ T cells promotes IL-6 driven Th17 cell responses in patients with T1R leprosy reactions.

Scientific reports

Saini C,Srivastava RK,Tarique M,Kurra S,Khanna N,Ramesh V,Sharma A

Tue Sep 15 00:00:00 EDT 2020

Transcriptional downregulation of MHC class I and melanoma de- differentiation in resistance to PD-1 inhibition.

Nature communications

Lee JH,Shklovskaya E,Lim SY,Carlino MS,Menzies AM,Stewart A,Pedersen B,Irvine M,Alavi S,Yang JYH,Strbenac D,Saw RPM,Thompson JF,Wilmott JS,Scolyer RA,Long GV,Kefford RF,Rizos H

Mon Apr 20 00:00:00 EDT 2020

DNA hypomethylation during MSC chondrogenesis occurs predominantly at enhancer regions.

Scientific reports

Barter MJ,Bui C,Cheung K,Falk J,Gómez R,Skelton AJ,Elliott HR,Reynard LN,Young DA

Fri Jan 24 00:00:00 EST 2020

A Non-canonical Pathway with Potential for Safer Modulation of Transforming Growth Factor-β1 in Steroid-Resistant Airway Diseases.

iScience

Li M,Keenan CR,Lopez-Campos G,Mangum JE,Chen Q,Prodanovic D,Xia YC,Langenbach SY,Harris T,Hofferek V,Reid GE,Stewart AG

AF-100-36E was used in Cell Culture to conclude that this pathway for glucocorticoids insensitivity offers several promising drug targets that potentially enable a safer approach to the modulation of TGF-β1 in chronic inflammatory diseases than is afforded by global TGF-β1 inhibition.

Fri Feb 22 00:00:00 EST 2019

Modeling Parkinson's disease in midbrain-like organoids.

NPJ Parkinson's disease

Smits LM,Reinhardt L,Reinhardt P,Glatza M,Monzel AS,Stanslowsky N,Rosato-Siri MD,Zanon A,Antony PM,Bellmann J,Nicklas SM,Hemmer K,Qing X,Berger E,Kalmbach N,Ehrlich M,Bolognin S,Hicks AA,Wegner F,Sterneckert JL,Schwamborn JC

Wed Sep 30 00:00:00 EDT 2020

Live Imaging Mitochondrial Transport in Neurons.

Neuromethods

Course MM,Hsieh CH,Tsai PI,Codding-Bui JA,Shaltouki A,Wang X

AF-100-36E was used in In vitro experiments to demonstrate imaging techniques to visualise mitochondrial transport system in neuronal networks, for physiological and pathological study applications.

Thu Oct 01 00:00:00 EDT 2020

Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3.

Scientific reports

He H,Kuriyan AE,Su CW,Mahabole M,Zhang Y,Zhu YT,Flynn HW,Parel JM,Tseng SC

AF-100-36E was used in In vitro experiments to investigate heavy chain-hyaluronic acid/pentraxin 3 inhibition of proliferative vitreoretinopathy.

Thu Mar 02 00:00:00 EST 2017

The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities.

PloS one

Chen X,Song F,Jhamb D,Li J,Bottino MC,Palakal MJ,Stocum DL

AF-100-36E was used in In vitro experiments to study the ability of the axolotl (Ambystoma mexicanum) fibula to regenerate across segment defects of different size in the absence of intervention or after implant of a unique 8-braid pig small intestine submucosa (SIS) scaffold, with or without incorporated growth factor combinations or tissue protein extract.

Mon Apr 25 00:00:00 EDT 2016

The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities.

PloS one

Chen X,Song F,Jhamb D,Li J,Bottino MC,Palakal MJ,Stocum DL

Mon Apr 25 00:00:00 EDT 2016

Lyn mitigates mouse airway remodeling by downregulating the TGF-β3 isoform in house dust mite models.

Journal of immunology (Baltimore, Md. : 1950)

Li G,Fox J,Liu Z,Liu J,Gao GF,Jin Y,Gao H,Wu M

Sun Dec 01 00:00:00 EST 2013

High glucose mediates endothelial-to-chondrocyte transition in human aortic endothelial cells.

Cardiovascular diabetology

Tang R,Gao M,Wu M,Liu H,Zhang X,Liu B

Sat Sep 22 00:00:00 EDT 2012

Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture.

PloS one

Krawetz RJ,Wu YE,Martin L,Rattner JB,Matyas JR,Hart DA

Tue Feb 19 00:00:00 EST 2013

TGF-β-operated growth inhibition and translineage commitment into smooth muscle cells of periodontal ligament-derived endothelial progenitor cells through Smad- and p38 MAPK-dependent signals.

International journal of biological sciences

Yoshida M,Okubo N,Chosa N,Hasegawa T,Ibi M,Kamo M,Kyakumoto S,Ishisaki A

Wed Nov 28 00:00:00 EST 2012

Crosstalk between nuclear factor I-C and transforming growth factor-β1 signaling regulates odontoblast differentiation and homeostasis.

PloS one

Lee DS,Yoon WJ,Cho ES,Kim HJ,Gronostajski RM,Cho MI,Park JC

Mon Aug 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: FLJ16571; H-TGF-b-3; prepro-transforming growth factor beta-3; tgf beta 3; TGF-beta-3; Transforming growth factor; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3

Human TGF-beta 3, Animal-Free Recombinant Protein, PeproTech®

Human TGF-beta 3, Animal-Free Protein (AF-100-36E-10UG) in FN

Product Details

AF-100-36E-10UG

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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