Recombinant TPO (Product # PHC9511) activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Product Details

PHC9514

Applications

Tested Dilution

Publications

Bioactivity (Bioactivity)

Assay-dependent

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Product Specifications

Species

Human

Published species

Human

Expression System

HEK293 cells

Molecular weight

85 kDa

Class

Recombinant

Type

Protein

Purity

>90% by SDS-PAGE

Endotoxin concentration

<1 EU/µg

Activity

ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Amount

10 µg

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Thrombopoietin in PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.

Storage: Store this lyophilized preparation at <-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at <-20°C. Avoid repeated freeze/thaw cycles.

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; ML; MPL ligand; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; Thrombopoietin; thrombopoietin nirs

Human TPO (Thrombopoietin) Recombinant Protein

Human TPO (Thrombopoietin) Protein (PHC9514) in Bioactivity