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Human TWEAK Recombinant Protein, PeproTech®

Human TWEAK Protein in Functional Assay (FN)

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Human TWEAK Protein (310-06-100UG) in FN

Bioassay analysis of Human TWEAK Recombinant Protein, PeproTech® (Product # 310-06-1MG).

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Product Details

310-06-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 15 publications 15 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH

Molecular weight

17 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nephrotoxicity of Calcineurin Inhibitors in Kidney Epithelial Cells is Independent of NFAT Signaling.

Frontiers in pharmacology

Karolin A,Escher G,Rudloff S,Sidler D

310-06 was used in Sample Preparation (FC/FACS) to study calcineurin inhibitors and their potential to induce nephrotoxicity in mice.

Wed Feb 16 00:00:00 EST 2022

Augmenting Immunotherapy Impact by Lowering Tumor TNF Cytotoxicity Threshold.

Cell

Vredevoogd DW,Kuilman T,Ligtenberg MA,Boshuizen J,Stecker KE,de Bruijn B,Krijgsman O,Huang X,Kenski JCN,Lacroix R,Mezzadra R,Gomez-Eerland R,Yildiz M,Dagidir I,Apriamashvili G,Zandhuis N,van der Noort V,Visser NL,Blank CU,Altelaar M,Schumacher TN,Peeper DS

Thu Jul 25 00:00:00 EDT 2019

Intestinal non-canonical NFκB signaling shapes the local and systemic immune response.

Nature communications

Ramakrishnan SK,Zhang H,Ma X,Jung I,Schwartz AJ,Triner D,Devenport SN,Das NK,Xue X,Zeng MY,Hu Y,Mortensen RM,Greenson JK,Cascalho M,Wobus CE,Colacino JA,Nunez G,Rui L,Shah YM

Fri Feb 08 00:00:00 EST 2019

Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins.

Nature communications

Winer H,Fraiberg M,Abada A,Dadosh T,Tamim-Yecheskel BC,Elazar Z

Fri Sep 14 00:00:00 EDT 2018

NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion.

Current biology : CB

Jung JU,Ravi S,Lee DW,McFadden K,Kamradt ML,Toussaint LG,Sitcheran R

310-06 was used in In vitro experiments to highlight the importance of NF-κB-inducing kinase (NIK) in tumor pathogenesis and invite new therapeutic strategies that attenuate mitochondrial dysfunction through inhibition of NIK and Drp1.

Mon Dec 19 00:00:00 EST 2016

NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway.

Oncogenesis

Duran CL,Lee DW,Jung JU,Ravi S,Pogue CB,Toussaint LG,Bayless KJ,Sitcheran R

Mon Jun 06 00:00:00 EDT 2016

Non-canonical NF-κB signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation.

Nature cell biology

Li Y,Zhou QL,Sun W,Chandrasekharan P,Cheng HS,Ying Z,Lakshmanan M,Raju A,Tenen DG,Cheng SY,Chuang KH,Li J,Prabhakar S,Li M,Tergaonkar V

310-06 was used in Cell Culture to highlight a previously unrecognized role of non-canonical NF-κB signalling in tumorigenesis and elucidate a fundamental mechanism for TERT reactivation in cancers, which if targeted could have immense therapeutic implications.

Thu Oct 01 00:00:00 EDT 2015

Mcl-1 mediates TWEAK/Fn14-induced non-small cell lung cancer survival and therapeutic response.

Molecular cancer research : MCR

Whitsett TG,Mathews IT,Cardone MH,Lena RJ,Pierceall WE,Bittner M,Sima C,LoBello J,Weiss GJ,Tran NL

Tue Apr 01 00:00:00 EDT 2014

The Src homology 3 domain-containing guanine nucleotide exchange factor is overexpressed in high-grade gliomas and promotes tumor necrosis factor-like weak inducer of apoptosis-fibroblast growth factor-inducible 14-induced cell migration and invasion via tumor necrosis factor receptor-associated factor 2.

The Journal of biological chemistry

Fortin Ensign SP,Mathews IT,Eschbacher JM,Loftus JC,Symons MH,Tran NL

Fri Jul 26 00:00:00 EDT 2013

TWEAK enhances E-selectin and ICAM-1 expression, and may contribute to the development of cutaneous vasculitis.

PloS one

Chen T,Guo ZP,Li L,Li MM,Wang TT,Jia RZ,Cao N,Li JY

Wed Aug 28 00:00:00 EDT 2013

TWEAK enhances E-selectin and ICAM-1 expression, and may contribute to the development of cutaneous vasculitis.

PloS one

Chen T,Guo ZP,Li L,Li MM,Wang TT,Jia RZ,Cao N,Li JY

Wed Aug 28 00:00:00 EDT 2013

Structural basis and targeting of the interaction between fibroblast growth factor-inducible 14 and tumor necrosis factor-like weak inducer of apoptosis.

The Journal of biological chemistry

Dhruv H,Loftus JC,Narang P,Petit JL,Fameree M,Burton J,Tchegho G,Chow D,Yin H,Al-Abed Y,Berens ME,Tran NL,Meurice N

Fri Nov 08 00:00:00 EST 2013

TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.

Journal of neuroinflammation

Stephan D,Sbai O,Wen J,Couraud PO,Putterman C,Khrestchatisky M,Desplat-Jégo S

Tue Jan 15 00:00:00 EST 2013

Functional expression of TWEAK and the receptor Fn14 in human malignant ovarian tumors: possible implication for ovarian tumor intervention.

PloS one

Gu L,Dai L,Cao C,Zhu J,Ding C,Xu HB,Qiu L,Di W

Tue Aug 27 00:00:00 EDT 2013

Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling.

Nature methods

Sims JJ,Scavone F,Cooper EM,Kane LA,Youle RJ,Boeke JD,Cohen RE

Sun Feb 05 00:00:00 EST 2012

A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications.

Journal of immunology (Baltimore, Md. : 1950)

Bover LC,Cardó-Vila M,Kuniyasu A,Sun J,Rangel R,Takeya M,Aggarwal BB,Arap W,Pasqualini R

Fri Jun 15 00:00:00 EDT 2007

The tumor necrosis factor-like weak inducer of apoptosis (TWEAK)-fibroblast growth factor-inducible 14 (Fn14) signaling system regulates glioma cell survival via NFkappaB pathway activation and BCL-XL/BCL-W expression.

The Journal of biological chemistry

Tran NL,McDonough WS,Savitch BA,Sawyer TF,Winkles JA,Berens ME

Fri Feb 04 00:00:00 EST 2005

The human Fn14 receptor gene is up-regulated in migrating glioma cells in vitro and overexpressed in advanced glial tumors.

The American journal of pathology

Tran NL,McDonough WS,Donohue PJ,Winkles JA,Berens TJ,Ross KR,Hoelzinger DB,Beaudry C,Coons SW,Berens ME

Tue Apr 01 00:00:00 EST 2003

Induction and intracellular regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mediated apotosis in human malignant glioma cells.

Cancer research

Hao C,Beguinot F,Condorelli G,Trencia A,Van Meir EG,Yong VW,Parney IF,Roa WH,Petruk KC

Thu Feb 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: APO3 ligand; APO3/DR3 ligand; MGC129581; MGC20669; TNF-related WEAK inducer of apoptosis; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; Tumor necrosis factor ligand superfamily member 12; TWEAK

Human TWEAK Recombinant Protein, PeproTech®

Human TWEAK Protein (310-06-100UG) in FN

Product Details

310-06-100UG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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