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Invitrogen
Human TYW1B (aa 160-238) Control Fragment Recombinant Protein
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Product Details
RP-103003
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
RVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHR
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (67%), Rat (67%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61415. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: long intergenic non-protein coding RNA 69; MGC87315; Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; radical S-adenosyl methionine and flavodoxin domains 1; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B; tRNA wybutosine-synthesizing protein 1 homolog B; tRNA-yW synthesizing protein 1 homolog B (non-protein coding)
Gene Aliases: LINC00069; NCRNA00069; RSAFD2; TYW1B
UniProt ID: (Human) Q6NUM6
Entrez Gene ID: (Human) 441250
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