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Invitrogen
Human ULK3, His Tag Recombinant Protein
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Product Details
PV6436
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
44-end
Tag
His-tag
Molecular weight
54.3 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Amount
10 µg
Purification
purified
Storage buffer
PBS, pH 7, with 150mM Imidazole, 0.1mM PMSF, 25% glycerol, 0.2mM DTT
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Target Information
ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZP434C131; EC:2.7.11.1; FLJ90566; Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3; unnamed protein product
Gene Aliases: ULK3
UniProt ID: (Human) Q6PHR2
Entrez Gene ID: (Human) 25989
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