Gibco

Human gAcrp30/Adipolean Recombinant Protein, PeproTech®

Human gAcrp30/Adipolean Protein in Functional Assay (FN)

FIGURE: 1 / 1

Human gAcrp30/Adipolean Protein (450-21-100UG) in FN

Bioassay analysis of Human gAcrp30/Adipolean Recombinant Protein, PeproTech® (Product # 450-21-1MG).

Product Image Gallery

Product Details

450-21-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (FN)

Assay-dependent

In vitro Assay (IV)

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN

Molecular weight

16.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<2 EU/µg

Activity

Determined by its ability to inhibit the proliferation of murine M1 cells. The expected ED50 for this effect is 1.0-3.0 ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

450-21-1MG will be provided as 2 x 500 µg (450-21-500UG).

Recombinant Human gAcrp30/Adipolean is a 16.6 kDa protein consisting of 145 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogramming.

Journal of experimental & clinical cancer research : CR

Pham DV,Park PH

450-21 was used in Cell Culture to elucidate the multifaceted role of adiponectin in tumor fatty acid metabolic reprogramming and provides evidence for the connection between its metabolic actions and suppression of breast cancer.

Wed Jan 05 00:00:00 EST 2022

Role of a Contactin multi-molecular complex secreted by oligodendrocytes in nodal protein clustering in the CNS.

Glia

Dubessy AL,Mazuir E,Rappeneau Q,Ou S,Abi Ghanem C,Piquand K,Aigrot MS,Thétiot M,Desmazières A,Chan E,Fitzgibbon M,Fleming M,Krauss R,Zalc B,Ranscht B,Lubetzki C,Sol-Foulon N

Sun Dec 01 00:00:00 EST 2019

Critical role of tristetraprolin and AU-rich element RNA-binding protein 1 in the suppression of cancer cell growth by globular adiponectin.

FEBS open bio

Tilija Pun N,Khakurel A,Shrestha A,Kim SH,Park PH

Sat Dec 01 00:00:00 EST 2018

Fish oil prevents high-saturated fat diet-induced impairments in adiponectin and insulin response in rodent soleus muscle.

American journal of physiology. Regulatory, integrative and comparative physiology

Tishinsky JM,Gulli RA,Mullen KL,Dyck DJ,Robinson LE

Thu Mar 01 00:00:00 EST 2012

Interleukin-1 receptor-associated kinase-3 is a key inhibitor of inflammation in obesity and metabolic syndrome.

PloS one

Hulsmans M,Geeraert B,De Keyzer D,Mertens A,Lannoo M,Vanaudenaerde B,Hoylaerts M,Benhabilès N,Tsatsanis C,Mathieu C,Holvoet P

Mon Jun 11 00:00:00 EDT 2012

Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.

PloS one

Hulsmans M,Van Dooren E,Mathieu C,Holvoet P

Mon Jul 23 00:00:00 EDT 2012

Adiponectin pretreatment counteracts the detrimental effect of a diabetic environment on endothelial progenitors.

Diabetes

Leicht SF,Schwarz TM,Hermann PC,Seissler J,Aicher A,Heeschen C

Tue Feb 01 00:00:00 EST 2011

Decreased adiponectin and increased inflammation expression in epicardial adipose tissue in coronary artery disease.

Cardiovascular diabetology

Zhou Y,Wei Y,Wang L,Wang X,Du X,Sun Z,Dong N,Chen X

Wed Jan 12 00:00:00 EST 2011

Adiponectin and heme oxygenase-1 suppress TLR4/MyD88-independent signaling in rat Kupffer cells and in mice after chronic ethanol exposure.

Journal of immunology (Baltimore, Md. : 1950)

Mandal P,Roychowdhury S,Park PH,Pratt BT,Roger T,Nagy LE

Fri Oct 15 00:00:00 EDT 2010

Adiponectin resistance precedes the accumulation of skeletal muscle lipids and insulin resistance in high-fat-fed rats.

American journal of physiology. Regulatory, integrative and comparative physiology

Mullen KL,Pritchard J,Ritchie I,Snook LA,Chabowski A,Bonen A,Wright D,Dyck DJ

Sun Feb 01 00:00:00 EST 2009

Adiponectin regulates albuminuria and podocyte function in mice.

The Journal of clinical investigation

Sharma K,Ramachandrarao S,Qiu G,Usui HK,Zhu Y,Dunn SR,Ouedraogo R,Hough K,McCue P,Chan L,Falkner B,Goldstein BJ

Thu May 01 00:00:00 EDT 2008

Adiponectin inhibits vascular endothelial growth factor-induced migration of human coronary artery endothelial cells.

Cardiovascular research

Mahadev K,Wu X,Donnelly S,Ouedraogo R,Eckhart AD,Goldstein BJ

Thu May 01 00:00:00 EDT 2008

Suppression of lipopolysaccharide-stimulated tumor necrosis factor-alpha production by adiponectin is mediated by transcriptional and post-transcriptional mechanisms.

The Journal of biological chemistry

Park PH,Huang H,McMullen MR,Mandal P,Sun L,Nagy LE

Fri Oct 03 00:00:00 EDT 2008

Adiponectin deficiency increases leukocyte-endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo.

The Journal of clinical investigation

Ouedraogo R,Gong Y,Berzins B,Wu X,Mahadev K,Hough K,Chan L,Goldstein BJ,Scalia R

Fri Jun 01 00:00:00 EDT 2007

Globular adiponectin resistance develops independently of impaired insulin-stimulated glucose transport in soleus muscle from high-fat-fed rats.

American journal of physiology. Endocrinology and metabolism

Mullen KL,Smith AC,Junkin KA,Dyck DJ

Sun Jul 01 00:00:00 EDT 2007

Short-term treatment of RAW264.7 macrophages with adiponectin increases tumor necrosis factor-alpha (TNF-alpha) expression via ERK1/2 activation and Egr-1 expression: role of TNF-alpha in adiponectin-stimulated interleukin-10 production.

The Journal of biological chemistry

Park PH,McMullen MR,Huang H,Thakur V,Nagy LE

Fri Jul 27 00:00:00 EDT 2007

The stimulatory effect of globular adiponectin on insulin-stimulated glucose uptake and fatty acid oxidation is impaired in skeletal muscle from obese subjects.

Diabetes

Bruce CR,Mertz VA,Heigenhauser GJ,Dyck DJ

Tue Nov 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; apM-1; gAcrp30; Gelatin-binding protein; gelatin-binding protein 28

Human gAcrp30/Adipolean Recombinant Protein, PeproTech®

Human gAcrp30/Adipolean Protein (450-21-100UG) in FN

Product Details

450-21-100UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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