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Invitrogen
IL-1 beta Recombinant Protein
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FIGURE: 1 / 1
IL-1 beta Protein (SIL1B) in ELISA Std
Sandwich ELISA analysis of human IL-1b was performed using a Human IL-1b Colorimetric ELISA Kit (Product # EH2IL1B) by loading 50 ul per well of Human IL-1b Recombinant Protein (Product # SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/ml across a 5 ug/ml mouse anti-human IL-1b (Product # M421B) pre-coated plate and incubating for 3 hours at room temperature. The plate was washed then incubated with 50 ul per well of a biotinylated-mouse anti-human IL-1b monoclonal antibody (Product # M420BB) in quadruplicate at 0.07 ug/ml fo... View More
Product Details
SIL1B
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Concentration
1 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
The human IL-1beta Standard is calibrated against WHO IL-1beta Reference Standard lot (8)6/522. 1 Thermo pg = 1 WHO pg = 0.1 WHO units.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at 2-8°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
1000 pg/ml when reconstituted at the recommend volume.
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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