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Invitrogen
IL-10 Recombinant Protein
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IL-10 Protein (SIL10) in ELISA Std
Sandwich ELISA analysis of human IL-10 was performed using a Human IL-10 Colorimetric ELISA kit (Product # EHIL10) by loading 50 ul per well of Human IL-10 recombinant protein (Product # SIL10) in triplicate at 600, 240, 96, 38.4, 15.36, and 0 pg/ml across a 2 ug/ml rat anti-human IL-10 monoclonal antibody (Product # M010) pre-coated plate and incubating for 1.5 hours at room temperature. The plate was washed, and incubated with 50 ul per well of an IL-10 biotinylated monoclonal antibody (Product # M011B) in triplicate at 0.3 ug/ml for 1.5... View More
Product Details
SIL10
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
1.5 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
The human IL-10 Standard is calibrated against WHO IL-10 Reference Standard lot #92/516. 1 Thermo pg = 1 WHO pg = 0.005 WHO units.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
1500 pg/ml when reconstituted at the recommend volume.
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
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