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Product Details
315-36-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Published species
Mouse
Expression System
E. coli
Amino acid sequence
SVRVEQVIKP KKNKTEGEKS TEKPKRKKKG GKNGKGRRNK KKKNPCTAKF QNFCIHGECR YIENLEVVTC NCHQDYFGER CGEKSMKTHS EDDKDLSK
Molecular weight
11.5 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Murine Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amphiregulin; AR; MGC13647; Schwannoma-derived growth factor; SDGF
Gene Aliases: AR; Areg; Mcub; Sdgf
UniProt ID: (Mouse) P31955
Entrez Gene ID: (Mouse) 11839
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