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Invitrogen
Mouse GM-CSF Recombinant Protein
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Product Details
14-8331-80
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
25 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ~14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.
Applications Reported: Recombinant mouse GM-CSF is biologically active and can promote proliferation of mouse MC/9 cells in culture. It is also used as a standard cytokine in mouse GM-CSF ELISA.
Applications Tested: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg.
Source: E. coli expressed mature mouse GM-CSF accession # NM_009969.
Bioactivity: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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