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Invitrogen
Mouse IL-21 Recombinant Protein
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Product Details
14-8211-80
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
25 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Mouse Interleukin-21 (IL-21) is a 146-amino acid protein with 57% identity to the human gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma.
Applications Reported: Recombinant mouse IL-21 is biologically active and has been reported for use in ELISA and bioassay.
Applications Tested: This IL-21 protein has been tested by bioassay, yielding an ED50 of 300 pg/mL, corresponding to a specific activity of approximately 3x10E6 Units/mg.
Source: E. coli expressed amino acids Pro25-Ser146 of mature mouse IL-21 accession # NM_021782.
Bioactivity: Measured by dose-dependent induction of proliferation of B9 cell line. The ED50 is typically 300 pg/mL, corresponding to a specific activity of 3 x 10E6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The DTT reduced protein migrates as a 15 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 15 kDa polypeptide.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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